A DNA helicase, powered by ATP hydrolysis, propels itself rapidly along one of the template DNA strands (here the lagging strand), forcing open the DNA helix ahead of the replication fork.

J-domain proteins (JDPs) coevolved with Hsp70s to trigger ATP hydrolysis and catalytically upload various substrate polypeptides in need to be structurally modified by the chaperone. Here, we measured ...

The motor domain contains the catalytic site for ATP hydrolysis and the actin or microtubule-binding site. The neck region acts as a lever arm, amplifying the small conformational changes generated by ...

they injected ATP into ClC-3 protein samples before freezing to reduce hydrolysis. Researchers performed all-atom molecular ...