“I then purified this plasmid cloning vector that had the gal operon and showed that I could take the purified DNA and reintroduce it back into E. coli using a method that [Stanford biochemist] Dale ...

which is the main delivery vehicle into the cell and contains an antibiotic resistance gene for plasmid selection. This process is referred to as cloning. It uses restriction enzymes to cut the ...

The pSM2 plasmid harbors a conditional bacterial origin ... bacterial clones for each individual target oligonucleotide when cloning individual shRNAs, or ensure at least threefold coverage ...

To prepare the cloning vectors, transform separately pET11a-link-NGFP and pMRBAD-link-CGFP into an E. coli strain that gives high-quality plasmid DNA (for example, DH10B or XL1-Blue) and plate ...

Discover the price reduction of ENFINIA™ DNA products by 25%, improving access to high-quality DNA for researchers.