gene point mutations—both directed to a specific nucleotide or randomly generated throughout the gene—gene cloning, plasmid and bacteriophage constructions.” A particularly useful ...

which is the main delivery vehicle into the cell and contains an antibiotic resistance gene for plasmid selection. This process is referred to as cloning. It uses restriction enzymes to cut the ...

During the webinar, you will learn about Elegen’s proprietary cell-free cloning technology for highly accurate fragments for plasmid construction, and how it is used for building and expressing ...

To prepare the cloning vectors, transform separately pET11a-link-NGFP and pMRBAD-link-CGFP into an E. coli strain that gives high-quality plasmid DNA (for example, DH10B or XL1-Blue) and plate ...